significance of measurement of delta bilirubin in different types of liver diseases
Arpita Bhakta,Kartik Maiti
Published in International Journal of Advanced Research in Biology and BioTechnology
ISSN: xxxx-xxx Impact Factor:xx Volume:1 Issue:1 Year: 10 March,2015 Pages:10-17
Abstract
Determination of serum bilirubin is one of the important liver function tests. Serum bilirubin has been differentially determined by diazo reagent as total bilirubin and direct bilirubin. Recently which involves a covalent bond to albumin has drawn attention because of clinical significance. There is a method to measure delta bilirubin levels in patients with acute liver disease to examine its clinical significance. For the measurement of delta bilirubin at first measure the total bilirubin, direct bilirubin from Vitros 250 Dry chemistry Method. So delta bilirubin correlated with total bilirubin and direct bilirubin. Hence we can conclude from this case study that delta bilirubin might have a significant role in the diagnosis of these three types of jaundice. Although it cannot be measured but can be calculated by Dry chemistry Auto analyzer, probably it may help in the differential diagnosis of different types of jaundice since its found to be highest delta bilirubin Percentage in Obstructive Jaundice.
Kewords
bilirubin, diazo reagent, delta bilirubin, Vitros 250, Obstructive Jaundice
Reference
1. Ahlfors, C. E. (1981) Effect of serum dilution on apparent unbound bilirubin concentration as measured by the peroxidise method. Clin. Chem. 27, 692–696. 2. Ahlfors, C. E., and DiBiasio-Erwin, D. (1986) Rate constants for dissociation of bilirubin from its binding sites in neonatal (cord) and adult sera. J. Pediatr. 108, 295–298. 3. American Academy of Pediatrics. Provisional Committee for Quality Improvement and Subcommittee on Hyperbilirubinemia (1994) Practice parameter: Management of hyperbilirubinemia in the healthy term newborn. Pediatrics 94, 558–565. 4. Brodersen, R. (1974) Competitive binding of bilirubin and drugs to human serum albumin studied by enzymatic oxidation. J. Clin. Invest. 54, 1353–1364. 5. Brown, A. K., and Johnson, L. L. (1996) in The Yearbook of Neonatal and Perinatal Medicine (Fanaroff, A. A., and Klaus, M. H., Eds.), pp. xvii–xxviii, Mosby-Year Book, Chicago. 6. Chen TS, Chen PS (1984) Understanding the Liver. A History. Westport, CT: Greenwood Press, p. 99. 7. Chen, R. F. (1973) in Fluorescence Techniques in Cell Biology (Thaer, A. A., and Sernetz, M., Eds.), pp. 273–282, Springer- Verlag, New York. 8. Come SE, Shohet SB, Robinson SH (1974) Surface remodeling vs. whole-cell hemolysis of reticulocytes produced with erythroid stimulation or iron deficiency anemia. Blood 44, 817–830. 9. Diamond, I., and Schmid, R. (1966) Experimental bilirubin encephalopathy. The mode of entry of bilirubin-14C into the central nervous system. J. Clin. Invest. 45, 678–689. 10. Faerch, T., and Jacobsen, J. (1975) Determination of association and dissociation rate constants for bilirubin–bovine serum albumin. Arch. Biochem. Biophys. 184, 351–357. 11. Funato, M., Tamai, H., Shimada, S., and Nakamura, H. (1994) Vigintiphobia, unbound bilirubin, and auditory brainstem responses. Pediatrics 93, 50–53. 12. Jacobsen, J., and Wennberg, R. P. (1974) Determination of unbound bilirubin in the serum of newborns. Clin. Chem. 20, 783–789. 13. Knudsen, E., Pedersen, A. O., and Brodersen, R. (1986) Spectroscopic properties of bilirubin–human serum albumin complexes: A stoichiometric analysis. Arch. Biochem. Biophys. 244, 273– 284. 14. Kodak Ektachem test methodology: BuBc test methodology (1986) Health Sciences Marketing Division Publication MP2-40. Eastman Kodak Company, Rochester, New York. 15. Lauff, J. J., Kasper, M. E., Wu, T. W., and Ambrose, R. T. (1982) Isolation and preliminary characterization of a fraction of bilirubin in serum that is firmly bound to protein. Clin. Chem. 28, 629–637. 16. London IM, West R, Shemin D et al. (1950) On the origin of bile pigment in normal man. J Biol Chem 184, 351–358. 17. Nakamura, H., Yonetani, M., Uetani, Y., Funato, M., and Lee, Y. (1992) Determination of serum unbound bilirubin for prediction of kernicterus in low birth weight infants. Acta Paediatr. Jpn. 34, 642–647. 18. Odell, G. B. (1959) Studies in kernicterus. I. The protein binding of bilirubin. J. Clin. Invest. 38, 823–833. 19. Polacek, K. (1966) Risk of kernicterus in newborn infants with a high level of conjugated bilirubin. Acta Paediatr. Scand. 55,401–404. 20. Robinson SH (1977) Origins of the early-labeled peak. In: Berk PD, Berlin NI (eds) Bile Pigments: Chemistry and Physiology. Washington, DC: US Government Printing Office, pp. 175–188.
